Borrelia morphotypes

Borrelia burgdorferi is a spirochete bacterium that causes tick-borne Lyme disease. In laboratory cultures, B. burgdorferi develops several pleomorphic forms known as morphotypes.

Functional and structural differences among these morphptypes have been studied, but the changes in their expression at the transcriptome level have never been investigated.

To address this problem, we raised B. burgdorferi  morphotype cultures in various  growing conditions, harvested their RNA and recovered transcriptomes by RNAseq profiling.

The aim of this project is to detect genes regulating transitions from one morphotype to another, describe differences between morphotypes on a molecular level and to find evolutionary imprints of differentially expressed genes specific for each morphotype by the usage of phylostratigraphic approach.

Our findings are a potential starting point for identifying genes responsible for virulence, antibiotic resistance and evasion of the immune system, making them targets for experimental validation and subsequent functional description, crucial when considering therapy strategies and diagnostic approaches.

Contact

Domazet-Lošo Research Group

Macroevolution Research. Our lab focuses on macro-evolutionary patterns across the tree of life.
Ruđer Bošković Institute

Division of Molecular Biology
Laboratory of Evolutionary Genetics
wing 5 / rooms 109-111

Biljenička cesta 54
HR-10000 Zagreb
Croatia
linkedin facebook pinterest youtube rss twitter instagram facebook-blank rss-blank linkedin-blank pinterest youtube twitter instagram