One of the most important advances in biofilm investigation during the last decades has been the availability to visualize living biofilms in 3D.
While today the vast majority of visualization methods inspects biofilms at the microscopic level, the macroscopic bacterial colony screening are considerably legging behind.
The necessity for adequate biofilm visuals becomes particularly important in studies grounded in long-lasting developmental experiments.
Using an interdisciplinary approach, we are developing an automated, sensitive time-lapse visualization method for investigating subtle changes in biofilm macrocolony development.
By combining morphological changes with transcriptomics data, we are addressing crucial questions in the evolutionary genetics of biofilms.